Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application
Description
- Tytuł: Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application
- Autor/Autorzy:
- Nazwa czasopisma: Journal of Functional Biomaterials
- Rok: 2021
- ISSN: 2079-4983
- DOI: 10.3390/jfb12020037
- Adres www:: https://www.mdpi.com/2079-4983/12/2/37
- Strony od-do: 1-17
- Język: angielski
- Abstrakt: Recently, the modification of the initial structure of biopolymers, mainly chitosan, has been gaining importance with a view to obtain functional forms with increased practicality and specific properties enabling their use in tissue engineering. Therefore, in this article, the properties (structural and biological) of thermosensitive hydrogels obtained from chitosan lactate/chloride and two types of crosslinking agents (β-glycerol phosphate disodium salt pentahydrate and uridine 5 0 -monophosphate disodium salt) are discussed. The aim of the research is to identify changes in the structure of the biomaterials during conditioning in water. Structural investigations were carried out by FTIR spectroscopy. The crystallinity of gels was determined by X-ray diffraction analysis. The biocompatibility (evaluation of cytotoxicity and genotoxicity) of chitosan hydrogels was investigated by contact with human colon adenocarcinoma cell line for 48 h. The cytotoxicity was verified based on the colorimetric resazurin assay, and the genotoxicity was checked by the comet assay (percentage of DNA in the comet tail). The conducted research showed that the analyzed types of chitosan hydrogels are non-cytotoxic and non-genotoxic materials. The good biocompatibility of chitosan hydrogels surfaces makes them interesting scaffolds with clinical potential in tissue regeneration engineering
- Dyscyplina: nauki medyczne
MARC
- 002 $a Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application
- 003 $b 0000-0001-8757-6958
- 003 $a EWA KUCHARSKA (Autor)
- 003 $a Grzegorz Galita (Autor)
- 003 $a Ireneusz Majsterek (Autor)
- 003 $a Katarzyna Pieklarz (Autor)
- 003 $a Michał Tylman (Autor)
- 003 $a Waldemar Maniukiewicz (Autor)
- 003 $a Zofia Modrzejewska (Autor)
- 004 $a Oryginalny artykuł naukowy
- 006 $a Journal of Functional Biomaterials
- 008 $a 2021
- 011 $a 2079-4983
- 013 $a 10.3390/jfb12020037
- 014 $a https://www.mdpi.com/2079-4983/12/2/37
- 015 $a 1-17
- 017 $a angielski
- 020 $a Recently, the modification of the initial structure of biopolymers, mainly chitosan, has been gaining importance with a view to obtain functional forms with increased practicality and specific properties enabling their use in tissue engineering. Therefore, in this article, the properties (structural and biological) of thermosensitive hydrogels obtained from chitosan lactate/chloride and two types of crosslinking agents (β-glycerol phosphate disodium salt pentahydrate and uridine 5 0 -monophosphate disodium salt) are discussed. The aim of the research is to identify changes in the structure of the biomaterials during conditioning in water. Structural investigations were carried out by FTIR spectroscopy. The crystallinity of gels was determined by X-ray diffraction analysis. The biocompatibility (evaluation of cytotoxicity and genotoxicity) of chitosan hydrogels was investigated by contact with human colon adenocarcinoma cell line for 48 h. The cytotoxicity was verified based on the colorimetric resazurin assay, and the genotoxicity was checked by the comet assay (percentage of DNA in the comet tail). The conducted research showed that the analyzed types of chitosan hydrogels are non-cytotoxic and non-genotoxic materials. The good biocompatibility of chitosan hydrogels surfaces makes them interesting scaffolds with clinical potential in tissue regeneration engineering
- 966 $a nauki medyczne
- 985 $a Wydział Pedagogiczny
- 985 $b Instytut Nauk o Wychowaniu
Dublin Core
Indexes
- Title: Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application
- Author:
- Journal: Journal of Functional Biomaterials
- Date: 2021
- Discipline: nauki medyczne
- Structure:
- Language: angielski